GMNN Monoclonal antibody
GMNN Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2E3A2
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, HEK-293 cells, NCCIT cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66566-1-Ig targets GMNN in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | rat |
| Immunogen | GMNN fusion protein Ag24283 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | geminin, DNA replication inhibitor |
| Synonyms | Gem, Geminin, GMNN, RP3 369A17.3 |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC005185 |
| Gene Symbol | GMNN |
| Gene ID (NCBI) | 51053 |
| RRID | AB_2881927 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75496 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GMNN antibody 66566-1-Ig | Download protocol |
| IHC protocol for GMNN antibody 66566-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex |
