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GPR155 Polyclonal antibody

GPR155 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12659-1-AP

Print datasheet

Synonyms

DEP.7, DEPDC3, G protein coupled receptor 155, GPR155, LYCHOS, PGR22

验证数据展示

  • WB analysis using 12659-1-AP

    WB analysis using 12659-1-AP

    mouse brain lysates were subjected to SDS PAGE followed by western blot with 12659-1-AP (GPR155 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12659-1-AP

    IP experiment of mouse brain using 12659-1-AP

    IP result of anti-GPR155 (IP:12659-1-AP, 4ug; Detection:12659-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  • IHC staining of human brain using 12659-1-AP

    IHC staining of human brain using 12659-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 12659-1-AP

    IHC staining of human brain using 12659-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12659-1-AP targets GPR155 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity human, mouse
Immunogen GPR155 fusion protein Ag3328 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 155
Synonyms DEP.7, DEPDC3, G protein coupled receptor 155, GPR155, LYCHOS, PGR22
Calculated Molecular Weight 870 aa, 97 kDa
Observed Molecular Weight 97 kDa, 75 kDa
GenBank Accession NumberBC028730
Gene Symbol GPR155
Gene ID (NCBI) 151556
RRIDAB_2877869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3F1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The G Protein-coupled receptor 155 (GPR155), also known as Lysosomal cholesterol signaling protein (LYCHOS), is a cholesterol receptor on the lysosome. GRR155 plays a role in the cholesterol-sensing lysosomal pathway and couples cholesterol concentration to MTORC1-dependent anabolic signaling (PMID: 36007018). GPR155 may represent a biomarker for diagnosing and predicting hematogenous metastasis of gastric cancer (PMID: 28165032).

实验方案

Product Specific Protocols
WB protocol for GPR155 antibody 12659-1-APDownload protocol
IHC protocol for GPR155 antibody 12659-1-APDownload protocol
IP protocol for GPR155 antibody 12659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols