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HARS Monoclonal antibody

HARS Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B7G2

Cat No : 67662-1-Ig

Print datasheet

Synonyms

FLJ20491, HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS

验证数据展示

  • WB analysis using 67662-1-Ig

    WB analysis using 67662-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67662-1-Ig (HARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 67662-1-Ig

    IF Staining of MCF-7 using 67662-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (67662-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, HEK-293 cells, HepG2 cells, HeLa cells
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67662-1-Ig targets HARS in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen HARS fusion protein Ag9224 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histidyl-tRNA synthetase
Synonyms FLJ20491, HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS
Calculated Molecular Weight 509 aa, 57 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC011807
Gene Symbol HARS
Gene ID (NCBI) 3035
RRIDAB_2882859
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP12081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

实验方案

Product Specific Protocols
WB protocol for HARS antibody 67662-1-IgDownload protocol
IF protocol for HARS antibody 67662-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols