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HIPK3 Polyclonal antibody

HIPK3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25107-1-AP

Print datasheet

Synonyms

ANPK, DYRK6, FIST, FIST3, HIPK3, Homolog of protein kinase YAK1, PKY, YAK1

验证数据展示

  • WB analysis using 25107-1-AP

    WB analysis using 25107-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25107-1-AP (HIPK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25107-1-AP

    IHC staining of human testis using 25107-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25107-1-AP (HIPK3 Antibody) at dilution of 1:2000 (under 10x lens).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25107-1-AP targets HIPK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HIPK3 fusion protein Ag18897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homeodomain interacting protein kinase 3
Synonyms ANPK, DYRK6, FIST, FIST3, HIPK3, Homolog of protein kinase YAK1, PKY, YAK1
Calculated Molecular Weight 1215 aa, 134 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC156873
Gene Symbol HIPK3
Gene ID (NCBI) 10114
RRIDAB_2879899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H422
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIPK3 antibody 25107-1-APDownload protocol
IHC protocol for HIPK3 antibody 25107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Aging (Albany NY)

Identification of HIPK3 as a potential biomarker and an inhibitor of clear cell renal cell carcinoma.

Authors - Wen Xiao
View Article
humanWB,IHC

Sci Rep

Overexpression of microRNA-205-5p promotes cholangiocarcinoma growth by reducing expression of homeodomain-interacting protein kinase 3

Authors - Aye Myat Mon
View Article