HMOX2 Polyclonal antibody

HMOX2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14817-1-AP

Print datasheet

Synonyms

heme oxygenase (decycling) 2, Heme oxygenase 2, HMOX2, HO 2, HO2



经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, mouse spleen tissue, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14817-1-AP targets HMOX2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen HMOX2 fusion protein Ag6575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heme oxygenase (decycling) 2
Synonyms heme oxygenase (decycling) 2, Heme oxygenase 2, HMOX2, HO 2, HO2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC002396
Gene Symbol HMOX2
Gene ID (NCBI) 3163
RRIDAB_2118843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heme oxygenase is the rate-limiting enzyme in the heme degradative pathway. There are 2 isozymic forms of heme oxygenase, an inducible heme oxygenase-1 (HMOX1) and a constitutive heme oxygenase-2 (HMOX2). Down-regulation of HMOX2 is associated with the increased expression of HMOX1 in human cell lines. HMOX2 is also named as HO-2 and could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

实验方案

Product Specific Protocols
WB protocol for HMOX2 antibody 14817-1-APDownload protocol
IHC protocol for HMOX2 antibody 14817-1-APDownload protocol
IF protocol for HMOX2 antibody 14817-1-APDownload protocol
IP protocol for HMOX2 antibody 14817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
ratWB

Acta Pharm Sin B

Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.

Authors - Dominik Bakalarz
humanWB

J Cell Sci

Role of epithelial sodium channels (ENaCs) in endothelial function.

Authors - Dongqing Guo
human,mouseWB,IHC

Oxid Med Cell Longev

Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion.

Authors - László Potor
ratWB

Cells

Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium Lesions.

Authors - Katarzyna Magierowska
ratWB

PLoS One

Carbon Monoxide (CO) Released from Tricarbonyldichlororuthenium (II) Dimer (CORM-2) in Gastroprotection against Experimental Ethanol-Induced Gastric Damage.

Authors - Katarzyna Magierowska