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HSD17B11 Polyclonal antibody

HSD17B11 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17301-1-AP

Print datasheet

Synonyms

17 beta HSD 11, 17 beta HSD XI, 17 BETA HSD11, 17 BETA HSDXI, 17betaHSD11, 17betaHSDXI, 17bHSD11, DHRS8, HSD17B11, PAN1B, RETSDR2, SDR16C2, UNQ207/PRO233

验证数据展示

  • WB analysis using 17301-1-AP

    WB analysis using 17301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17301-1-AP (HSD17B11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17301-1-AP

    IHC staining of human kidney using 17301-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 17301-1-AP

    IHC staining of human kidney using 17301-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse pancreas tissue, rat pancreas，
Positive IHC detected inhuman kidney tissue, human brain tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17301-1-AP targets HSD17B11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HSD17B11 fusion protein Ag11182 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hydroxysteroid (17-beta) dehydrogenase 11
Synonyms 17 beta HSD 11, 17 beta HSD XI, 17 BETA HSD11, 17 BETA HSDXI, 17betaHSD11, 17betaHSDXI, 17bHSD11, DHRS8, HSD17B11, PAN1B, RETSDR2, SDR16C2, UNQ207/PRO233
Calculated Molecular Weight 300 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC016367
Gene Symbol HSD17B11
Gene ID (NCBI) 51170
RRIDAB_2119810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBQ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSD17B11 antibody 17301-1-APDownload protocol
IHC protocol for HSD17B11 antibody 17301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Syst Biol Reprod Med

Identification of ferroptotic genes and phenotypes in idiopathic nonobstructive azoospermia

Authors - Chen Liao
View Article