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HSPB11 Polyclonal antibody

HSPB11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 15732-1-AP

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Synonyms

C1orf41, Heat shock protein beta 11, HSPB11, HSPCO34, IFT25, ITF25, Placental protein 25, PP25



经过测试的应用

Positive WB detected inA549 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15732-1-AP targets HSPB11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen HSPB11 fusion protein Ag8490 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock protein family B (small), member 11
Synonyms C1orf41, Heat shock protein beta 11, HSPB11, HSPCO34, IFT25, ITF25, Placental protein 25, PP25
Calculated Molecular Weight 144 aa, 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC005245
Gene Symbol HSPB11
Gene ID (NCBI) 51668
RRIDAB_2279933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y547
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.

实验方案

Product Specific Protocols
WB protocol for HSPB11 antibody 15732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Authors - Malavika Raman
  • KD Validated
mouseWB,IF

Dev Cell

IFT25 links the signal-dependent movement of Hedgehog components to intraflagellar transport.

Authors - Keady Brian T BT
mouseWB,IF

Dev Cell

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.

Authors - Thibaut Eguether
mouseWB

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
mouseWB

Curr Biol

Rab34 is necessary for early stages of intracellular ciliogenesis.

Authors - Michael W Stuck
humanWB

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie