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HSPH1 Monoclonal antibody

HSPH1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H5A5

Cat No : 66723-1-Ig

Print datasheet

Synonyms

Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25

验证数据展示

  • WB analysis using 66723-1-Ig

    WB analysis using 66723-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66723-1-Ig (HSPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 66723-1-Ig

    IHC staining of mouse testis using 66723-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66723-1-Ig (HSPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66723-1-Ig

    IF Staining of HeLa using 66723-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66723-1-Ig (HSPH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66723-1-Ig targets HSPH1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen HSPH1 fusion protein Ag27521 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 105kDa/110kDa protein 1
Synonyms Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25
Calculated Molecular Weight 858 aa, 97 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC037553
Gene Symbol HSPH1
Gene ID (NCBI) 10808
RRIDAB_2882074
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92598
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. Western blot analysis using this antibody detected the phosphorylated protein around 110 kDa in various lysates.

实验方案

Product Specific Protocols
WB protocol for HSPH1 antibody 66723-1-IgDownload protocol
IHC protocol for HSPH1 antibody 66723-1-IgDownload protocol
IF protocol for HSPH1 antibody 66723-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols