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  • Featured Product
  • KD/KO Validated

HTATSF1 Polyclonal antibody

HTATSF1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20805-1-AP

Print datasheet

Synonyms

HIV 1 Tat specific factor 1, HIV Tat specific factor 1, HTATSF1, TAT SF1

验证数据展示

  • WB analysis of HeLa using 20805-1-AP

    WB analysis of HeLa using 20805-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 20805-1-AP

    IHC staining of human breast cancer using 20805-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with HTATSF1 antibody included
  • IF Staining of HeLa using 20805-1-AP

    IF Staining of HeLa using 20805-1-AP

    Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HepG2 using 20805-1-AP

    IP experiment of HepG2 using 20805-1-AP

    IP result of anti-HTATSF1 (IP:20805-1-AP, 4ug; Detection:20805-1-AP 1:2000) with HepG2 cells lysate 2400ug.

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, rat brain tissue, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy HTATSF1 Ready-To-Use IHC Kit. HTATSF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20805-1-AP is HTATSF1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HTATSF1 fusion protein Ag14744 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HIV-1 Tat specific factor 1
Synonyms HIV 1 Tat specific factor 1, HIV Tat specific factor 1, HTATSF1, TAT SF1
Calculated Molecular Weight 755 aa, 86 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC009896
Gene Symbol HTATSF1
Gene ID (NCBI) 27336
RRIDAB_10695767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43719
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HTATSF1 antibody 20805-1-APDownload protocol
IHC protocol for HTATSF1 antibody 20805-1-APDownload protocol
IF protocol for HTATSF1 antibody 20805-1-APDownload protocol
IP protocol for HTATSF1 antibody 20805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Authors - Jiao Zhao
  • KD Validated
View Article

Nucleic Acids Res

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Authors - Xiaodong Lu
View Article
humanWB,IHC,IP

Cell Death Discov

Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.

Authors - Yu-Chan Chang
  • KD Validated
View Article