Hemopexin Monoclonal antibody
Hemopexin Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, pig and More (1)
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3A9D6
验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, human placenta tissue, rat serum tissue, pig plasma tissue |
| Positive IHC detected in | human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human liver cancer tissue |
| Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HPX Ready-To-Use IHC Kit. HPX primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:40000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
66479-1-Ig targets Hemopexin in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, rat, pig samples.
| Tested Applications | FC, IF-P, IHC, WB, ELISA |
| Cited Applications | IF |
| Tested Reactivity | Human, rat, pig |
| Cited Reactivity | human |
| Immunogen | Hemopexin fusion protein Ag8533 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | hemopexin |
| Synonyms | Beta 1B glycoprotein, hemopexin, HPX |
| Calculated Molecular Weight | 254 aa, 29 kDa |
| Observed Molecular Weight | 65-75 kDa |
| GenBank Accession Number | BC005395 |
| Gene Symbol | HPX |
| Gene ID (NCBI) | 3263 |
| RRID | AB_2881845 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02790 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Hemopexin antibody 66479-1-Ig | Download protocol |
| IHC protocol for Hemopexin antibody 66479-1-Ig | Download protocol |
| IF protocol for Hemopexin antibody 66479-1-Ig | Download protocol |
| FC protocol for Hemopexin antibody 66479-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. |
