KE00132 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The AFP ELISA kit is to be used to detect and quantify protein levels of endogenous AFP. The assay recognizes human AFP. A monoclonal antibody specific for AFP has been pre-coated onto the microwells. The AFP protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for AFP is added to detect the captured AFP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.