Human APCS ELISA Kit0 Publications

Catalog number: KE00122

规格

单价

96 T

¥2600

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Overview


Product name:
Human APCS ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, plasma, Cell culture supernatants, Urine

Assay type:
Sandwich

Sensitivity:
2.6 pg/mL

Range:
15.6 - 1000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human plasma 104% 90%-121%
Human urine 103% 75%-119%
Cell culture supernatants 94% 81%-126%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 24.0 2.60 10.9
2 20 100.4 7.14 7.1
3 20 432.7 18.18 4.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 28.9 2.87 9.9
2 24 106.2 10.06 9.5
3 24 457.7 45.04 9.8

Product overview:
KE00122 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APCS ELISA kit is to be used to detect and quantify protein levels of endogenous APCS. The assay recognizes human APCS. A monoclonal antibody specific for APCS has been pre-coated onto the microwells. The APCS protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for APCS is added to detect the captured APCS protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
9.5S alpha 1 glycoprotein, amyloid P component, serum, APCS, PTX2, SAP, Serum amyloid P component
Background

Serum amyloid P component (SAP, also known as APCS), belongs to the family of pentraxins, a superfamily of plasma proteins characterized by their pentameric assembly and calcium-dependent ligand binding. SAP is a constitutive serum protein synthesized by hepatocytes. It is structurally related to C-reactive protein, a major acute-phase reactant in humans. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. SAP can also bind to fibrils in all types of amyloid deposits, and contributes to the pathogenesis of amyloidosis.


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