Human BMP7 ELISA Kit0 Publications

Catalog number: KE00127

规格

单价

96 T

¥2600

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Overview


Product name:
Human BMP7 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Cell culture supernatants, Urine

Assay type:
Sandwich

Sensitivity:
0.8 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human serum 90% 71%-121%
Cell culture supernatants 95% 74%-123%
Urine 97% 73%-118%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 517.2 30.8 5.9
2 20 125.8 6.6 5.3
3 20 30.7 2.2 7.1

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 399.7 37.7 9.4
2 24 99.5 7.8 7.8
3 24 27.3 2.1 7.7

Product overview:
KE00127 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BMP7 ELISA kit is to be used to detect and quantify protein levels of endogenous BMP7. The assay recognizes human BMP7. A monoclonal antibody specific for BMP7 has been pre-coated onto the microwells. The BMP7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for human BMP7 is added to detect the captured human BMP7 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
BMP 7, BMP7, bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, Osteogenic protein 1
Background

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 is a glycosylated disulfide-linked 36 kDa homodimeric protein, having role in the induction, development, and regulation of adipocytes, especially brown adipose tissue (BAT). BMP7 is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation. Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival. Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC.


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