Human CD146, MCAM ELISA Kit0 Publications

Catalog number: KE00065

规格

单价

96 T

¥2600

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Overview


Product name:
Human CD146, MCAM ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
3.8 ng/mL

Range:
62.5-4000 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Cell culture supernatants 95% 80%-125%
Human plasma 92% 79%-121%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 2,643.0 252.8 9.6
2 20 487.4 40.3 8.3
3 20 124.3 7.3 5.9

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 1,647.6 156.9 9.5
2 24 344.9 27.6 8.0
3 24 82.4 8.1 9.8

Product overview:
KE00065 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MCAM ELISA kit is to be used to detect and quantify protein levels of endogenous MCAM. The assay recognizes human MCAM. A monoclonal antibody specific for MCAM has been pre-coated onto the microwells. The MCAM protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody specific for MCAM is added to detect the captured MCAM protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
CD146, MCAM, MUC18
Background

N/A


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