Human CD147 ELISA Kit0 Publications

Catalog number: KE00135

规格

单价

96 T

¥2600

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Overview


Product name:
Human CD147 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Human plasma, Human serum, Cell culture supernatants, Human milk, Human urine, Human Saliva

Assay type:
Sandwich

Sensitivity:
2.1 pg/mL

Range:
31.25-2000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human plasma 101% 75-121
Human serum 97% 76-118
Cell culture supernatants 93% 80-115
Human milk 104% 88-125
Human urine 104% 78-121
Human Saliva 97% 84-118

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 56.5 1.1 2.0
2 20 219.7 6.7 3.1
3 20 816.5 33.5 4.1

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 56.2 2.5 4.4
2 24 221.9 9.6 4.3
3 24 865.6 58.0 6.7

Product overview:
KE00135 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD147 ELISA kit is to be used to detect and quantify protein levels of endogenous CD147. The assay recognizes human CD147. A polyclonal antibody specific for CD147 has been pre-coated onto the microwells. The CD147 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for human CD147 is added to detect the captured human CD147 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF
Background

N/A


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