Human CD23,FCER2 ELISA Kit0 Publications

Catalog number: KE00107

规格

单价

96 T

¥2600

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Overview


Product name:
Human CD23,FCER2 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant, Urine

Assay type:
Sandwich

Sensitivity:
2.2 pg/mL

Range:
31.25-2000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Urine 106% 96%-122%
Human plasma 101% 87%-119%
Cell culture supernatant 104% 82%-117%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 879.9 41.2 4.7
2 20 216.9 9.9 4.6
3 20 49.9 9.7 9.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 849.4 72.4 8.5
2 24 209.6 18.5 8.8
3 24 48.2 4.1 8.6

Product overview:
KE00107 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD23 ELISA kit is to be used to detect and quantify protein levels of endogenous CD23. The assay recognizes human CD23. A monoclonal antibody specific for CD23 has been pre-coated onto the microwells. The CD23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody specific for CD23 is added to detect the captured CD23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor
Background

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. CD23 can be cleaved into soluble fragment (soluble CD23, sCD23) of various sizes displaying pleiotropic biological activities. Increased plasma levels of sCD23 have been reported in allergic disorders, acute viral diseases, lepromatous leprosy, B-CLL, and inflammatory diseases like RA, SLE and sarcoidosis.


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