Human Clusterin ELISA Kit0 Publications

Catalog number: KE00110

规格

单价

96 T

¥2600

Images See all

Overview


Product name:
Human Clusterin ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants, Urine, Saliva

Assay type:
Sandwich

Sensitivity:
5.0 pg/mL

Range:
62.5-4000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human serum 112% 91%-118%
Cell culture supernatants 96% 72%-121%
Urine 99% 85%-123%
Saliva 99% 80%-112%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,832.2 52.3 2.9
2 20 451.4 12.3 2.7
3 20 109.4 5.0 4.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1,771.9 96.8 5.5
2 24 427.4 22.9 5.3
3 24 95.8 8.2 8.5

Product overview:
KE00110 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Clusterin ELISA kit is to be used to detect and quantify protein levels of endogenous Clusterin. The assay recognizes human Clusterin. A monoclonal antibody specific for Clusterin has been pre-coated onto the microwells. The Clusterin protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for human Clusterin is added to detect the captured human Clusterin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2
Background

Clusterin (also known as apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids. It is encoded by one single gene and cleaved posttranslationally into α and β subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement. Increased plasma clusterin levels have been associated with severity, pathology, and progression in Alzheimer disease.


Back
to top