Human GKN1 ELISA Kit

Sensitivity

5 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat No : KE00029

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Synonyms

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11



Product Information

KE00029 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GKN1 ELISA kit is to be used to detect and quantify protein levels of endogenous GKN1. The assay recognizes human GKN1. A polyclonal antibody specific for GKN1 has been pre-coated onto the microwells. The GKN1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for GKN1 is added to detect the captured GKN1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human GKN1 ELISA Kit
Tests 1 X 96 well plate
Sample type Plasma, Serum
Assay type Sandwich
Sensitivity 5 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 56287

Recovery

Sample TypeAverageRange
Plasma95%85%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

InterAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

Background Information

Gastrokine 1 (GKN1), a stomach-speci?c protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene is localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. As a secreted protein, GKN1 is specifically expressed in gastric mucosa to protect and maintain the integrity of gastric epithelium. GKN1 is involved in the replenishment of the surface lumen epithelial cell layer, and could play a role in cell proliferation and differentiation. Recent findings showed that GKN1 may act as a functional gastric tumor suppressor and may also modulate apoptotic signals.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms 18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11