Product name:

Human IFNA1 ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma

Assay type:

Sandwich

Sensitivity:

1.5 pg/mL

Range:

15.6-1000 pg/mL

Reacted Species:

Human

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Human plasma	102%	78%-121%

Product overview:

KE00044 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFNA1 ELISA kit is to be used to detect and quantify protein levels of endogenous IFNA1. The assay recognizes human IFNA1. A polyclonal antibody specific for IFNA1 has been pre-coated onto the microwells. The IFNA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IFNA1 is added to detect the captured IFNA1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Storage Instructions:

All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:

IFL, IFN, IFN ALPHA, IFN alpha 1/13, IFNA@, IFNA1, IFNA13, Interferon alpha 1/13, Interferon alpha D, interferon, alpha 1, LeIF D