Human IL28B ELISA Kit

Catalog number: KE00014

规格

单价

96 T

¥2600

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Overview


Product name:
Human IL28B ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.8 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 103% 86%-122%
Cell culture supernatant 97% 85%-113%

Product overview:
KE00014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL28B ELISA kit is to be used to detect and quantify protein levels of endogenous IL28B. The assay recognizes human IL28B. A polyclonal antibody specific for IL28B has been pre-coated onto the microwells. The IL28B protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL28B is added to detect the captured IL28B protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
IFN-lambda-3, Interferon lambda-3, Cytokine Zcyto22, Interleukin-28B, Interleukin-28C, IFNL3
Background

IL28B, also named as IFNL3, is a cytokine distantly related to type I interferons and the IL-10 family. IL28B, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).


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