Huntingtin Monoclonal antibody

Huntingtin Monoclonal Antibody for FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6C9

Cat No : 66553-1-Ig

Print datasheet

Synonyms

HD, HD protein, HTT, huntingtin, Huntington disease protein, IT15



经过测试的应用

Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy HTT Ready-To-Use IHC Kit. HTT primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66553-1-Ig targets Huntingtin in IHC, FC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications FC, IHC, ELISA
Tested Reactivity Human, Mouse
Immunogen Huntingtin fusion protein Ag25922 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name huntingtin
Synonyms HD, HD protein, HTT, huntingtin, Huntington disease protein, IT15
Calculated Molecular Weight 348 kDa
GenBank Accession NumberNM_002111
Gene Symbol HTT
Gene ID (NCBI) 3064
RRIDAB_2881915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Huntingtin antibody 66553-1-IgDownload protocol
FC protocol for Huntingtin antibody 66553-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols