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ICAM3 Polyclonal antibody

ICAM3 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17699-1-AP

Print datasheet

Synonyms

CD50, CDW50, ICAM 3, ICAM R, ICAM3

验证数据展示

  • WB analysis of Jurkat using 17699-1-AP

    WB analysis of Jurkat using 17699-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17699-1-AP

    IHC staining of human colon using 17699-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 17699-1-AP

    IHC staining of human colon using 17699-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of Jurkat using 17699-1-AP

    IP experiment of Jurkat using 17699-1-AP

    IP result of anti-ICAM3 (IP:17699-1-AP, 4ug; Detection:17699-1-AP 1:500) with Jurkat cells lysate 4000 ug.

  • FC experiment of Jurkat using 17699-1-AP

    FC experiment of Jurkat using 17699-1-AP

    1X10^6 Jurkat cells were stained with 0.5ug ICAM3 antibody (17699-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inJurkat cells, Ramos cells, U-937 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17699-1-AP targets ICAM3 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen ICAM3 fusion protein Ag11955 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intercellular adhesion molecule 3
Synonyms CD50, CDW50, ICAM 3, ICAM R, ICAM3
Calculated Molecular Weight 547 aa, 60 kDa
Observed Molecular Weight 110-160 kDa, 60 kDa
GenBank Accession NumberBC058903
Gene Symbol ICAM3
Gene ID (NCBI) 3385
RRIDAB_2248763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ICAM3 antibody 17699-1-APDownload protocol
IHC protocol for ICAM3 antibody 17699-1-APDownload protocol
IP protocol for ICAM3 antibody 17699-1-APDownload protocol
FC protocol for ICAM3 antibody 17699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols