IFIH1/MDA5 Monoclonal antibody

IFIH1/MDA5 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3D8B11

Cat No : 66770-1-Ig

Print datasheet

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116



经过测试的应用

Positive WB detected inMDA-MB-468 cells, HepG2 cells, THP-1 cells, Jurkat cells, Daudi cells, Raji cells, T-47D cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66770-1-Ig targets IFIH1/MDA5 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity Human
Cited Reactivityhuman, pig
Immunogen IFIH1/MDA5 fusion protein Ag17448 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interferon induced with helicase C domain 1
Synonyms Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116
Calculated Molecular Weight 1025 aa, 117 kDa
Observed Molecular Weight 117-125 kDa
GenBank Accession NumberBC111750
Gene Symbol IFIH1
Gene ID (NCBI) 64135
RRIDAB_2882116
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BYX4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).

实验方案

Product Specific Protocols
WB protocol for IFIH1/MDA5 antibody 66770-1-IgDownload protocol
IHC protocol for IFIH1/MDA5 antibody 66770-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Virol Sin

PRRSV degrades MDA5 via dual autophagy receptors P62 and CCT2 to evade antiviral innate immunity

Authors - Ruiqi Sun
  • KO Validated
humanWB

J Virol

Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2.

Authors - Yumei Han
humanWB

Vet Microbiol

DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation

Authors - Shujuan Xu
humanWB

Virus Res

Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro.

Authors - Qian Li
pigWB

Front Immunol

Temporally integrated transcriptome analysis reveals ASFV pathology and host response dynamics

Authors - Lin Lv
humanWB,IF

PLoS Pathog

SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination

Authors - Xiaolin Zhang