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  • KD/KO Validated

IFIT5 Polyclonal antibody

IFIT5 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13378-1-AP

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Synonyms

IFIT 5, IFIT5, RI58



经过测试的应用

Positive WB detected inA375 cells, PC-3 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13378-1-AP targets IFIT5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, pig, canine
Immunogen IFIT5 fusion protein Ag4202 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon-induced protein with tetratricopeptide repeats 5
Synonyms IFIT 5, IFIT5, RI58
Calculated Molecular Weight 482 aa, 58 kDa
Observed Molecular Weight 56-58 kDa, 48 kDa
GenBank Accession NumberBC025786
Gene Symbol IFIT5
Gene ID (NCBI) 24138
RRIDAB_10640300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13325
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.

实验方案

Product Specific Protocols
WB protocol for IFIT5 antibody 13378-1-APDownload protocol
IHC protocol for IFIT5 antibody 13378-1-APDownload protocol
IP protocol for IFIT5 antibody 13378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

IFIT5 Negatively Regulates the Type I IFN Pathway by Disrupting TBK1-IKKε-IRF3 Signalosome and Degrading IRF3 and IKKε.

Authors - Na Zhang
humanWB

Cell Signal

IFIT5 positively regulates NF-κB signaling through synergizing the recruitment of IκB kinase (IKK) to TGF-β-activated kinase 1 (TAK1).

Authors - Caishang Zheng
  • KD Validated
HumanWB

Acta Biochim Biophys Sin (Shanghai)

IFIT5 potentiates anti-viral response through enhancing innate immune signaling pathways.

Authors - Zhang Bianhong B
humanWB,IHC

Cell Death Dis

The roles and mechanism of IFIT5 in bladder cancer epithelial-mesenchymal transition and progression.

Authors - Jun Huang
humanIHC

Oncoimmunology

Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.

Authors - Chen Zhu
humanWB

mBio

PKR Binds Enterovirus IRESs, Displaces Host Translation Factors, and Impairs Viral Translation to Enable Innate Antiviral Signaling.

Authors - Mikhail I Dobrikov