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IL-10 Monoclonal antibody

IL-10 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (3)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

8E4B6

Cat No : 60269-1-Ig

Print datasheet

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



经过测试的应用

Positive WB detected inag14870
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMOLT-4 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60269-1-Ig targets IL-10 in WB, IHC, IF, Cell treatment, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications Cell treatment, ELISA, IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, rat, rabbit, zebrafish
Immunogen IL-10 fusion protein Ag14870 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin 10
Synonyms CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC104252
Gene Symbol IL10
Gene ID (NCBI) 3586
RRIDAB_2881389
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22301
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

实验方案

Product Specific Protocols
WB protocol for IL-10 antibody 60269-1-IgDownload protocol
IHC protocol for IL-10 antibody 60269-1-IgDownload protocol
IF protocol for IL-10 antibody 60269-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

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A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds

Authors - Shunlai Shang
mouseWB

Adv Healthc Mater

One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing.

Authors - Shen Guo
mouseWB

Mater Today Bio

Injectable multifunctional CMC/HA-DA hydrogel for repairing skin injury.

Authors - Longlong Cui
humanIHC

J Immunother Cancer

Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma.

Authors - Yuwei Deng
mouseWB

Mol Ther

Host liver-derived extracellular vesicles deliver miR-142a-3p induces neutrophil extracellular traps via targeting WASL to block the development of Schistosoma japonicum.

Authors - Lifu Wang
mouseWB,IF

Int J Biol Macromol

Repairing gastric ulcer with hyaluronic acid/extracellular matrix composite through promoting M2-type polarization of macrophages

Authors - Renhao Ni