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IL-11RA Polyclonal antibody

IL-11RA Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10264-1-AP

Print datasheet

Synonyms

IL 11 receptor subunit alpha, IL 11R alpha, IL 11R subunit alpha, IL 11RA, IL11RA, IL-11RA, interleukin 11 receptor, alpha

验证数据展示

  • WB analysis using 10264-1-AP

    WB analysis using 10264-1-AP

    Mouse colon tissue was subjected to SDS PAGE followed by western blot with 10264-1-AP (IL-11RA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of N2a cells using 10264-1-AP

    IF Staining of N2a cells using 10264-1-AP

    IF result of anti-IL11RA (10264-1-AP, 1:200) with N2a cells (4%PFA fixed) by Miss Masue Marbiah, PhD student MRC Prion Unit, Department of Neurodegenerative Diseases, UCL Institute of Neurology.

  • IHC staining of human prostate cancer using 10264-1-AP

    IHC staining of human prostate cancer using 10264-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 10264-1-AP

    IHC staining of human prostate cancer using 10264-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 10264-1-AP

    FC experiment of K-562 using 10264-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human IL-11RA (10264-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman prostate cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inN2a cells
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10264-1-AP targets IL-11RA in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen IL-11RA fusion protein Ag0373 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 11 receptor, alpha
Synonyms IL 11 receptor subunit alpha, IL 11R alpha, IL 11R subunit alpha, IL 11RA, IL11RA, IL-11RA, interleukin 11 receptor, alpha
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC003110
Gene Symbol IL11RA
Gene ID (NCBI) 3590
RRIDAB_2125660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-11 (IL-11), a member of the IL-6 family of cytokines, was firstly identified in bone marrow-derived stromal cells (PMID: 2145578). IL-11 is a multifunctional cytokine that plays important roles in hematopoiesis, bone development, tissue repair, and tumor development (PMID: 33863879). IL-10 signals through a receptor complex consisting of IL-11 receptor alpha subunit (IL-11RA) and gp130. IL-11RA binds IL-11 and gp130 transmits signals to the nucleus via Janus kinase (JAK) activation. In addition to a membrane-bound form, IL-11RA can also exist as a soluble form (sIL-11RA) that acts as an agonist of IL-11 activity (PMID: 26876177; 27493449).

实验方案

Product Specific Protocols
WB protocol for IL-11RA antibody 10264-1-APDownload protocol
IHC protocol for IL-11RA antibody 10264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

EMBO J

Identification of a gene regulatory network associated with prion replication.

Authors - Masue M Marbiah
View Article
mouseWB

ACS Nano

Nanoparticle-Mediated RNA Therapy Attenuates Nonalcoholic Steatohepatitis and Related Fibrosis by Targeting Activated Hepatic Stellate Cells

Authors - Chenshuang Zhang
View Article