- Featured Product
- KD/KO Validated
IL-6 Polyclonal antibody
IL-6 Polyclonal Antibody for FC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
FC, WB, ELISA and More (4)
Conjugate
Unconjugated
534
验证数据展示
经过测试的应用
Positive WB detected in | LPS and Protein transport inhibitor treated HUVEC cells |
Positive FC detected in | LPS and Brefeldin A treated HUVEC cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21865-1-AP targets IL-6 in WB, IF, FC, IHC, ELISA, Neutralization, Cell treatment applications and shows reactivity with human samples.
Tested Applications | FC, WB, ELISA |
Cited Applications | WB, IF, IHC, ELISA, Neutralization, Cell treatment |
Tested Reactivity | human |
Cited Reactivity | human, goat, pig, canine, bovine |
Immunogen | IL-6 fusion protein Ag10565 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | interleukin 6 (interferon, beta 2) |
Synonyms | B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6 |
Calculated Molecular Weight | 212 aa, 24 kDa |
Observed Molecular Weight | 20 kDa |
GenBank Accession Number | BC015511 |
Gene Symbol | IL6 |
Gene ID (NCBI) | 3569 |
ENSEMBL Gene ID | ENSG00000136244 |
RRID | AB_11142677 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P05231 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IL-6 antibody 21865-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Cancer Cell Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation. | ||
Bioact Mater A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds | ||
ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | ||
Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation | ||
Mol Ther Epsin1-mediated exosomal sorting of Dll4 modulates the tubular-macrophage crosstalk in diabetic nephropathy |