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ING2 Monoclonal antibody

ING2 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C3A7

Cat No : 66512-1-Ig

Print datasheet

Synonyms

ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2

验证数据展示

  • WB analysis of HSC-T6 using 66512-1-Ig

    WB analysis of HSC-T6 using 66512-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 66512-1-Ig

    FC experiment of HepG2 using 66512-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human ING2 (66512-1-Ig, Clone:2C3A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHSCT6 cells, T-47D cells, NIH/3T3 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ING2 Ready-To-Use IHC Kit. ING2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66512-1-Ig targets ING2 in WB, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC, WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen ING2 fusion protein Ag19291 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name inhibitor of growth family, member 2
Synonyms ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2
Calculated Molecular Weight 280 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC030128
Gene Symbol ING2
Gene ID (NCBI) 3622
RRIDAB_2881875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H160
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program.

实验方案

Product Specific Protocols
WB protocol for ING2 antibody 66512-1-IgDownload protocol
FC protocol for ING2 antibody 66512-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols