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ING3-specific Polyclonal antibody

ING3-specific Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 16187-1-AP

Print datasheet

Synonyms

Eaf4, ING2, ING3, p47ING3

验证数据展示

  • WB analysis of human kidney using 16187-1-AP

    WB analysis of human kidney using 16187-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 16187-1-AP

    IP experiment of mouse kidney using 16187-1-AP

    IP result of anti-ING3-specific (IP:16187-1-AP, 4ug; Detection:16187-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman kidney tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16187-1-AP targets ING3-specific in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inhibitor of growth family, member 3
Synonyms Eaf4, ING2, ING3, p47ING3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberNM_019071
Gene Symbol ING3
Gene ID (NCBI) 54556
RRIDAB_2264993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXR8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.

实验方案

Product Specific Protocols
WB protocol for ING3-specific antibody 16187-1-APDownload protocol
IP protocol for ING3-specific antibody 16187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols