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Integrin Alpha 3 Polyclonal antibody

Integrin Alpha 3 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21992-1-AP

Print datasheet

Synonyms

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

验证数据展示

  • WB analysis of A549 using 21992-1-AP

    WB analysis of A549 using 21992-1-AP

    WB result of Integrin Alpha 3 antibody (21992-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells.

  • WB analysis using 21992-1-AP

    WB analysis using 21992-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 21992-1-AP

    IHC staining of mouse colon using 21992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ITGA3 antibody included
  • IHC staining of mouse colon using 21992-1-AP

    IHC staining of mouse colon using 21992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ITGA3 antibody included
  • IP experiment of PC-3 using 21992-1-AP

    IP experiment of PC-3 using 21992-1-AP

    IP result of anti-Integrin Alpha 3 (IP:21992-1-AP, 4ug; Detection:21992-1-AP 1:500) with PC-3 cells lysate 1200ug.

  • IF Staining of A549 using 21992-1-AP

    IF Staining of A549 using 21992-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Integrin Alpha 3 antibody (21992-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of PC-3 using 21992-1-AP

    FC experiment of PC-3 using 21992-1-AP

    1X10^6 PC-3 cells were stained with 0.2ug Integrin alpha-3 antibody (21992-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inA549 cells, human heart tissue, human testis tissue, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy ITGA3 Ready-To-Use IHC Kit. ITGA3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21992-1-AP targets Integrin Alpha 3 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Integrin Alpha 3 fusion protein Ag17293 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
Synonyms CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a
Calculated Molecular Weight 1066 aa, 119 kDa
Observed Molecular Weight 130-160 kDa
GenBank Accession NumberBC150190
Gene Symbol ITGA3
Gene ID (NCBI) 3675
RRIDAB_10953527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26006
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

实验方案

Product Specific Protocols
WB protocol for Integrin Alpha 3 antibody 21992-1-APDownload protocol
IHC protocol for Integrin Alpha 3 antibody 21992-1-APDownload protocol
IF protocol for Integrin Alpha 3 antibody 21992-1-APDownload protocol
IP protocol for Integrin Alpha 3 antibody 21992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

High Expression of Integrin α3 Predicts Poor Prognosis and Promotes Tumor Metastasis and Angiogenesis by Activating the c-Src/Extracellular Signal-Regulated Protein Kinase/Focal Adhesion Kinase Signaling Pathway in Cervical Cancer.

Authors - Qiqiao Du
View Article
human,mouseWB,IF

Nat Commun

Integrin α3β1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosis.

Authors - Eunnyung Bae
View Article