Involucrin Polyclonal antibody
Involucrin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HT-1080 cells, HT-29 cells |
Positive IHC detected in | human tonsillitis tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
Positive FC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28462-1-AP targets Involucrin in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Immunogen | Involucrin fusion protein Ag29585 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | involucrin |
Synonyms | involucrin, IVL |
Calculated Molecular Weight | 68 kDa |
Observed Molecular Weight | 120 kDa |
GenBank Accession Number | NM_005547 |
Gene Symbol | IVL |
Gene ID (NCBI) | 3713 |
RRID | AB_2881148 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P07476 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Involucrin antibody 28462-1-AP | Download protocol |
IHC protocol for Involucrin antibody 28462-1-AP | Download protocol |
IF protocol for Involucrin antibody 28462-1-AP | Download protocol |
FC protocol for Involucrin antibody 28462-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Nanobiotechnology Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration. | ||
Int Immunopharmacol Nicotinamide mononucleotide ameliorates DNFB-induced atopic dermatitis-like symptoms in mice by blocking activation of ROS-mediated JAK2/STAT5 signaling pathway. | ||
J Ethnopharmacol Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes. | ||
J Oncol Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma | ||
Biol Pharm Bull Pyridoxine Has a Potential to Prevent the Appearance of Pigmented Spots: Effects on the Phagocytosis and Differentiation of Keratinocytes | ||
Front Pharmacol Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation |