Involucrin Polyclonal antibody

Involucrin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28462-1-AP

Print datasheet

Synonyms

involucrin, IVL



经过测试的应用

Positive WB detected inA431 cells, HT-1080 cells, HT-29 cells
Positive IHC detected inhuman tonsillitis tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28462-1-AP targets Involucrin in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen Involucrin fusion protein Ag29585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name involucrin
Synonyms involucrin, IVL
Calculated Molecular Weight 68 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberNM_005547
Gene Symbol IVL
Gene ID (NCBI) 3713
RRIDAB_2881148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07476
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

实验方案

Product Specific Protocols
WB protocol for Involucrin antibody 28462-1-APDownload protocol
IHC protocol for Involucrin antibody 28462-1-APDownload protocol
IF protocol for Involucrin antibody 28462-1-APDownload protocol
FC protocol for Involucrin antibody 28462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Nanobiotechnology

Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.

Authors - Yunyao Bo
mouseIF

Int Immunopharmacol

Nicotinamide mononucleotide ameliorates DNFB-induced atopic dermatitis-like symptoms in mice by blocking activation of ROS-mediated JAK2/STAT5 signaling pathway.

Authors - Jie-Fang Gao
mouseWB,IHC

J Ethnopharmacol

Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.

Authors - Zhenzhuo Li
humanWB

J Oncol

Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma

Authors - Shiyu Zeng
humanWB

Biol Pharm Bull

Pyridoxine Has a Potential to Prevent the Appearance of Pigmented Spots: Effects on the Phagocytosis and Differentiation of Keratinocytes

Authors - Yushi Katsuyama
human,mouseWB

Front Pharmacol

Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation

Authors - Lusheng Wang