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  • Featured Product
  • KD/KO Validated

KIAA1279 Polyclonal antibody

KIAA1279 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25653-1-AP

Print datasheet

Synonyms

KBP, KIAA1279, KIF1 binding protein, TTC20

验证数据展示

  • WB analysis using 25653-1-AP

    WB analysis using 25653-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 25653-1-AP

    IHC staining of mouse kidney using 25653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, NIH/3T3 cells
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25653-1-AP targets KIAA1279 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen KIAA1279 fusion protein Ag22234 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1279
Synonyms KBP, KIAA1279, KIF1 binding protein, TTC20
Calculated Molecular Weight 621 aa, 72 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC012180
Gene Symbol KIAA1279
Gene ID (NCBI) 26128
RRIDAB_2880178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIAA1279 antibody 25653-1-APDownload protocol
IHC protocol for KIAA1279 antibody 25653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Mol Neurosci

Multiple Functions of KBP in Neural Development Underlie Brain Anomalies in Goldberg-Shprintzen Syndrome.

Authors - Hsin-Yun Chang
  • KD Validated
View Article
mouseWB, IF

Reproduction

KIF1-binding protein interacts with KIF3A in haploid male germ cells.

Authors - Mari S Lehti
View Article