KLK3/PSA Polyclonal antibody

KLK3/PSA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10679-1-AP

Print datasheet

Synonyms

APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, KLK3/PSA, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin



经过测试的应用

Positive WB detected inLNCaP cells, Transfected HEK-293 cells
Positive IP detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inLNCaP cells
Planning an IHC experiment? We recommend our IHCeasy PSA Ready-To-Use IHC Kit. PSA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10679-1-AP targets KLK3/PSA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen KLK3/PSA fusion protein Ag1060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kallikrein-related peptidase 3
Synonyms APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, KLK3/PSA, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC005307
Gene Symbol KLK3
Gene ID (NCBI) 354
RRIDAB_2134244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07288
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IHC protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IF protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IP protocol for KLK3/PSA antibody 10679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Theranostics

ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.

Authors - Lijie Zhou
mouseIHC

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
humanWB

Elife

A feedback loop between the androgen receptor and 6-phosphogluoconate dehydrogenase (6PGD) drives prostate cancer growth

Authors - Joanna L Gillis
humanWB

Mol Cancer Ther

PMID: 28802253

Authors - Linh N K Tran
mouseWB,IF

Stem Cell Res Ther

Expansion of mouse castration-resistant intermediate prostate stem cells in vitro

Authors - Yalan Xu
humanWB

Cancer Metab

Prostate cancer cell proliferation is influenced by LDL-cholesterol availability and cholesteryl ester turnover.

Authors - Nikki L Raftopulos