Cytokeratin 17 Polyclonal antibody
Cytokeratin 17 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells |
Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 22230-1-AP is Cytokeratin 17 Fusion Protein expressed in E. coli.
Tested Applications | IF, IHC, WB, ELISA |
Tested Reactivity | human, mouse |
Immunogen | Cytokeratin 17 fusion protein Ag17575 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | keratin 17 |
Synonyms | 39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1 |
Calculated Molecular Weight | 432 aa, 48 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | BC000159 |
Gene Symbol | KRT17 |
Gene ID (NCBI) | 3872 |
RRID | AB_2879040 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q04695 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 17 antibody 22230-1-AP | Download protocol |
IHC protocol for Cytokeratin 17 antibody 22230-1-AP | Download protocol |
IF protocol for Cytokeratin 17 antibody 22230-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |