Cytokeratin 17 Polyclonal antibody

Cytokeratin 17 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22230-1-AP

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Synonyms

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1



经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT17 Ready-To-Use IHC Kit. KRT17 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22230-1-AP is Cytokeratin 17 Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen Cytokeratin 17 fusion protein Ag17575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 17
Synonyms 39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000159
Gene Symbol KRT17
Gene ID (NCBI) 3872
RRIDAB_2879040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
IHC protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
IF protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols