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  • KD/KO Validated

LACTB Polyclonal antibody

LACTB Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 18195-1-AP

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Synonyms

G24, lactamase, beta, LACTB, MRPL56



经过测试的应用

Positive WB detected inHepG2 cells, human heart tissue, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18195-1-AP targets LACTB in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen LACTB fusion protein Ag12233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lactamase, beta
Synonyms G24, lactamase, beta, LACTB, MRPL56
Calculated Molecular Weight 547 aa, 61 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC067288
Gene Symbol LACTB
Gene ID (NCBI) 114294
RRIDAB_2280998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP83111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LACTB is also named as MRPL56,which is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form. This antibody is specific to LACTB.

实验方案

Product Specific Protocols
WB protocol for LACTB antibody 18195-1-APDownload protocol
IHC protocol for LACTB antibody 18195-1-APDownload protocol
IF protocol for LACTB antibody 18195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Nature

LACTB is a tumour suppressor that modulates lipid metabolism and cell state.

Authors - Zuzana Keckesova
humanWB,IHC,IF

Oncogene

LACTB, a novel epigenetic silenced tumor suppressor, inhibits colorectal cancer progression by attenuating MDM2-mediated p53 ubiquitination and degradation.

Authors - Kaixuan Zeng
  • KO Validated
HumanIHC

PLoS One

LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis.

Authors - Jian Xie
humanIHC

Cancer Biother Radiopharm

Clinical Significance of β-Lactamase Expression in Colorectal Cancer.

Authors - Shaofei Zhou
humanWB

Exp Ther Med

LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway.

Authors - Yihui Xu
  • KD Validated
humanWB,IF

Apoptosis

LACTB induces cancer cell death through the activation of the intrinsic caspase-independent pathway in breast cancer

Authors - Juan M Gonzalez-Morena