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LRCH3 Polyclonal antibody

LRCH3 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15800-1-AP

Print datasheet

Synonyms

LRCH3

验证数据展示

  • WB analysis of human liver using 15800-1-AP

    WB analysis of human liver using 15800-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 15800-1-AP

    IP experiment of mouse liver using 15800-1-AP

    IP result of anti-LRCH3 (IP:15800-1-AP, 3ug; Detection:15800-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

  • IF Staining of A431 using 15800-1-AP

    IF Staining of A431 using 15800-1-AP

    Immunofluorescent analysis of A431 cells using 15800-1-AP (LRCH3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman liver tissue, human heart tissue, human spleen tissue, Jurkat cells, mouse lung tissue
Positive IP detected inmouse liver tissue
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15800-1-AP targets LRCH3 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen LRCH3 fusion protein Ag8511 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine-rich repeats and calponin homology (CH) domain containing 3
Synonyms LRCH3
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007504
Gene Symbol LRCH3
Gene ID (NCBI) 84859
RRIDAB_2138461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96II8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRCH3 antibody 15800-1-APDownload protocol
IF protocol for LRCH3 antibody 15800-1-APDownload protocol
IP protocol for LRCH3 antibody 15800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols