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LRRC18 Polyclonal antibody

LRRC18 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24571-1-AP

Print datasheet

Synonyms

LRRC18, UNQ933, UNQ9338, VKGE9338

验证数据展示

  • WB analysis of mouse testis using 24571-1-AP

    WB analysis of mouse testis using 24571-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24571-1-AP (LRRC18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 24571-1-AP

    IP experiment of mouse testis using 24571-1-AP

    IP result of anti-LRRC18 (IP:24571-1-AP, 3ug; Detection:24571-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • IHC staining of human testis using 24571-1-AP

    IHC staining of human testis using 24571-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24571-1-AP (LRRC18 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24571-1-AP is LRRC18 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human, mouse
Immunogen LRRC18 fusion protein Ag20996 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 18
Synonyms LRRC18, UNQ933, UNQ9338, VKGE9338
Calculated Molecular Weight 261 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC036722
Gene Symbol LRRC18
Gene ID (NCBI) 474354
RRIDAB_2879614
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N456
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine rich repeat containing protein 18 ( LRRC18 ) encodes a 261 amino-acids protein (29.6 kDa), which contains 7 leucine rich repeat and belongs to LRR superfamily. The function of protein is still non-known, but it may be involved in the regulation of spermatogenesis and sperm maturation. The protein localizes in cytoplasm and express in some male reproduction tissues.

实验方案

Product Specific Protocols
WB protocol for LRRC18 antibody 24571-1-APDownload protocol
IHC protocol for LRRC18 antibody 24571-1-APDownload protocol
IP protocol for LRRC18 antibody 24571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols