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MAG Monoclonal antibody

MAG Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

3H9G3

Cat No : 66709-1-Ig

Print datasheet

Synonyms

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

验证数据展示

  • WB analysis of rat brain using 66709-1-Ig

    WB analysis of rat brain using 66709-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66709-1-Ig

    IHC staining of mouse brain using 66709-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66709-1-Ig targets MAG in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen MAG fusion protein Ag6065 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myelin associated glycoprotein
Synonyms GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC053347
Gene Symbol MAG
Gene ID (NCBI) 4099
RRIDAB_2882061
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

实验方案

Product Specific Protocols
WB protocol for MAG antibody 66709-1-IgDownload protocol
IHC protocol for MAG antibody 66709-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols