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  • KD/KO Validated

CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17564-1-AP

Print datasheet

Synonyms

CD146, MCAM, MUC18



经过测试的应用

Positive WB detected inHeLa cells, A375 cells, mouse lung tissue, HUVEC cells, HepG2 cells
Positive IP detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, HeLa cells
Positive FC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17564-1-AP targets CD146/MCAM in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsCell treatment, FC, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CD146/MCAM fusion protein Ag11762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma cell adhesion molecule
Synonyms CD146, MCAM, MUC18
Calculated Molecular Weight 646 aa, 72 kDa
Observed Molecular Weight 113-120 kDa
GenBank Accession NumberBC056418
Gene Symbol MCAM
Gene ID (NCBI) 4162
RRIDAB_2143373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

实验方案

Product Specific Protocols
WB protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IHC protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IF protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IP protocol for CD146/MCAM antibody 17564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Biomaterials

Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.

Authors - W Benton Swanson
ratIF

Adv Healthc Mater

Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration

Authors - Xiaoyi Wu
humanWB

Oncogene

The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.

Authors - M Sechler
  • KD Validated
humanIF

Cell Prolif

Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.

Authors - Lihua Luo
humanFC

Stem Cell Res Ther

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Authors - Zuping Wu
ratWB

J Proteome Res

Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.

Authors - Ji Yuhua Y
  • KD Validated