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MDMX Polyclonal antibody

MDMX Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17914-1-AP

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Synonyms

Double minute 4 protein, HDMX, Mdm2 like p53 binding protein, MDM4, MDMX, MRP1, p53 binding protein Mdm4, Protein Mdm4, Protein Mdmx



经过测试的应用

Positive WB detected inHeLa cells, Raji cells, HepG2 cells, Apoptosised HeLa cells, A375 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17914-1-AP targets MDMX in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MDMX fusion protein Ag12319 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Mdm4 p53 binding protein homolog (mouse)
Synonyms Double minute 4 protein, HDMX, Mdm2 like p53 binding protein, MDM4, MDMX, MRP1, p53 binding protein Mdm4, Protein Mdm4, Protein Mdmx
Calculated Molecular Weight 490 aa, 55 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC067299
Gene Symbol MDM4
Gene ID (NCBI) 4194
RRIDAB_2143683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MDMX antibody 17914-1-APDownload protocol
IHC protocol for MDMX antibody 17914-1-APDownload protocol
IP protocol for MDMX antibody 17914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Brain

Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

Authors - Hyojung Kim
humanWB

Cancer Res

FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX.

Authors - Xiang Ling
ratWB

Redox Biol

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Authors - Yuehong Wang
humanWB

Cell Death Discov

LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX

Authors - Qingqing Zhao
humanWB

J Med Chem

Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.

Authors - Shiyan Zhang
mouseWB

PLoS Genet

MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.

Authors - Meenalakshmi Chinnam
  • KO Validated