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  • KD/KO Validated

MFF Polyclonal antibody

MFF Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17090-1-AP

Print datasheet

Synonyms

C2orf33, MFF, mitochondrial fission factor



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17090-1-AP targets MFF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, monkey, hamster, squirrel
Immunogen MFF fusion protein Ag9990 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial fission factor
Synonyms C2orf33, MFF, mitochondrial fission factor
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC000797
Gene Symbol MFF
Gene ID (NCBI) 56947
RRIDAB_2142463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

实验方案

Product Specific Protocols
WB protocol for MFF antibody 17090-1-APDownload protocol
IHC protocol for MFF antibody 17090-1-APDownload protocol
IF protocol for MFF antibody 17090-1-APDownload protocol
IP protocol for MFF antibody 17090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Multiple dynamin family members collaborate to drive mitochondrial division.

Authors - Jason E Lee
human

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
mouseWB

Nature

Correcting mitochondrial fusion by manipulating mitofusin conformations.

Authors - Antonietta Franco
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
monkeyWB

Science

ER tubules mark sites of mitochondrial division.

Authors - Jonathan R Friedman
  • KD Validated
humanWB

Cell

CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity.

Authors - Philipp Hammerschmidt