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  • Featured Product
  • KD/KO Validated

MRPL4 Polyclonal antibody

MRPL4 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27484-1-AP

Print datasheet

Synonyms

CGI 28, L4mt, MRP L4, MRPL4

验证数据展示

  • WB analysis of HL-60 using 27484-1-AP

    WB analysis of HL-60 using 27484-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 27484-1-AP (MRPL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27484-1-AP

    IHC staining of human liver using 27484-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27484-1-AP (MRPL4 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27484-1-AP

    IHC staining of human liver using 27484-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27484-1-AP (MRPL4 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 27484-1-AP

    IF Staining of HepG2 using 27484-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 27484-1-AP (MRPL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:40000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27484-1-AP targets MRPL4 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited Applications WB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen MRPL4 fusion protein Ag26568 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L4
Synonyms CGI 28, L4mt, MRP L4, MRPL4
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000756
Gene Symbol MRPL4
Gene ID (NCBI) 51073
RRIDAB_2810294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYD3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL4 antibody 27484-1-APDownload protocol
IHC protocol for MRPL4 antibody 27484-1-APDownload protocol
IF protocol for MRPL4 antibody 27484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Metab

Tetracyclines promote survival and fitness in mitochondrial disease models.

Authors - Elizabeth A Perry
  • KO Validated
View Article
humanWB

Nucleic Acids Res

CoLoC-seq probes the global topology of organelle transcriptomes

Authors - Damien Jeandard
View Article
humanWB

bioRxiv

Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival

Authors - Conor T Ronayne
View Article
humanWB

EMBO Rep

Tetracyclines activate mitoribosome quality control and reduce ER stress to promote cell survival

Authors - Conor T Ronayne
View Article