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MTAP Monoclonal antibody

MTAP Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B1G6

Cat No : 66706-1-Ig

Print datasheet

Synonyms

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

验证数据展示

  • WB analysis of NIH/3T3 using 66706-1-Ig

    WB analysis of NIH/3T3 using 66706-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 66706-1-Ig

    IHC staining of mouse liver using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66706-1-Ig

    IF Staining of HeLa using 66706-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTAP antibody (66706-1-Ig, Clone: 2B1G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HCT 116 cells, HSC-T6 cells, Raji cells, HT-29 cells
Positive IHC detected inmouse liver tissue, human kidney tissue, rat liver tissue, mouse kidney tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66706-1-Ig targets MTAP in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen MTAP fusion protein Ag17954 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methylthioadenosine phosphorylase
Synonyms c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase
Calculated Molecular Weight 283 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
RRIDAB_2882058
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

实验方案

Product Specific Protocols
WB protocol for MTAP antibody 66706-1-IgDownload protocol
IHC protocol for MTAP antibody 66706-1-IgDownload protocol
IF protocol for MTAP antibody 66706-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols