MYC-tag Antibody 15 Publications

Mouse Monoclonal| Catalog number: 66004-1-Ig |CloneNo.: 2D11A8

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Species specificity:
Recombinant protein

Positive WB detected in:
Recombinant protein

Positive IP detected in:
Transfected HEK-293 cells

Positive IF detected in:
Transfected HEK-293 cells

Recommended dilution:
WB : 1:2000-1:20000
IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
IF : 1:10-1:100

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
MYC-tag

Calculated molecular weight:

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
EQKLISEEDL, MYC tag
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.


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