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MYH10 Polyclonal antibody

MYH10 Polyclonal Antibody for FC, IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 21403-1-AP

Print datasheet

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

验证数据展示

  • WB analysis using 21403-1-AP

    WB analysis using 21403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21403-1-AP (MYH10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21403-1-AP

    IF Staining of HepG2 using 21403-1-AP

    Immunofluorescent analysis of HepG2 cells, using MYH10 antibody 21403-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • FC experiment of HepG2 using 21403-1-AP

    FC experiment of HepG2 using 21403-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human MYH10 (21403-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

  • IP experiment of mouse brain using 21403-1-AP

    IP experiment of mouse brain using 21403-1-AP

    IP result of anti-MYH10 (IP:21403-1-AP, 4ug; Detection:21403-1-AP 1:1500) with mouse brain tissue lysate 3440ug.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain
Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21403-1-AP is MYH10 Fusion Protein expressed in E. coli.

Tested Applications FC, IF/ICC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen MYH10 fusion protein Ag16113 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, heavy chain 10, non-muscle
Synonyms MYH10, Myosin 10, Myosin heavy chain 10
Calculated Molecular Weight 1985 aa, 230 kDa
Observed Molecular Weight 229 kDa
GenBank Accession NumberBC150634
Gene Symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_10804351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35580
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

实验方案

Product Specific Protocols
WB protocol for MYH10 antibody 21403-1-APDownload protocol
IF protocol for MYH10 antibody 21403-1-APDownload protocol
IP protocol for MYH10 antibody 21403-1-APDownload protocol
FC protocol for MYH10 antibody 21403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols