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MYH10-Specific Polyclonal antibody

MYH10-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19673-1-AP

Print datasheet

Synonyms

MYH10, MYH10-Specific, Myosin 10, Myosin heavy chain 10



经过测试的应用

Positive WB detected inHEK-293T cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19673-1-AP targets MYH10-Specific in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsCoIP, ELISA, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, heavy chain 10, non-muscle
Synonyms MYH10, MYH10-Specific, Myosin 10, Myosin heavy chain 10
Calculated Molecular Weight 231 kDa
Observed Molecular Weight 229 kDa
GenBank Accession NumberNM_005964
Gene Symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_10858231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35580
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.

实验方案

Product Specific Protocols
WB protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IHC protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IF protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IP protocol for MYH10-Specific antibody 19673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan
ratWB,CoIP

Bioact Mater

Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.

Authors - Pengzhen Cheng
humanWB

J Am Heart Assoc

Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.

Authors - Jiaqi Tang
humanIHC

Clin Genet

Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.

Authors - Zhongxian Zhu
mouseWB

Sci Rep

Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease.

Authors - Jeong Suk Kang
humanWB

J Dermatol Sci

Decreased mitochondrial function in UVA-irradiated dermal fibroblasts causes the insufficient formation of type I collagen and fibrillin-1 fibers

Authors - Yushi Katsuyama