MYH2-specific Polyclonal antibody

MYH2-specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 55069-1-AP

Print datasheet

Synonyms

MYH2, MYH2A, MYHas8, MyHC 2A, MyHC IIa, MYHSA2, Myosin 2, Myosin 2a, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa



经过测试的应用

Positive WB detected inmulti-cells/tissue, human skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55069-1-AP targets MYH2-specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA
Cited Applications WB
Tested Reactivity human
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, heavy chain 2, skeletal muscle, adult
Synonyms MYH2, MYH2A, MYHas8, MyHC 2A, MyHC IIa, MYHSA2, Myosin 2, Myosin 2a, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa
Calculated Molecular Weight 223 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberNM_017534
Gene Symbol MYH2
Gene ID (NCBI) 4620
RRIDAB_10860267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKX2
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.

实验方案

Product Specific Protocols
WB protocol for MYH2-specific antibody 55069-1-APDownload protocol
IHC protocol for MYH2-specific antibody 55069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle

Authors - Xueqiao Wang