Myosin Light Chain 2/MLC-2V Polyclonal antibody

Myosin Light Chain 2/MLC-2V Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (2)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10906-1-AP

Print datasheet

Synonyms

MLC 2, MLC 2v, MLC2, MLC2V, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V



经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10906-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications FC, IF, IHC, WB
Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, monkey, zebrafish, pig
Immunogen Myosin Light Chain 2/MLC-2V fusion protein Ag1356 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, light chain 2, regulatory, cardiac, slow
Synonyms MLC 2, MLC 2v, MLC2, MLC2V, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC015821
Gene Symbol MYL2
Gene ID (NCBI) 4633
RRIDAB_2147453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Background

MYL2 belongs to the myosin family of motor proteins. Their common features are ATP hydrolysis, actin binding, and potential for kinetic energy transduction. MYL2 stands for Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2), also known as the regulatory light chain of myosin (RLC). This isoform is distinct from those expressed in skeletal muscle (MYLPF), smooth muscle (MYL12B), and cardiac atrial muscle (MYL7) (PMID: 21345328). Due to its tissue specificity, MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

 What is the molecular weight of MYL2?

There are two isoforms of this protein in humans that differ only slightly in length and produce proteins composed of 152 and 166 aa, which correspond to about 19 kDa (PMID: 16678204). 

 What is the subcellular localization of MYL2?

 It is uniquely expressed in the cytoplasm of heart muscles. It colocalizes with actin cytoskeleton staining.

 What is the tissue specificity of MYL2?

 MYL2 is specifically expressed in ventricular cardiac tissue.

 What is the function of MYL2 in cardiac tissue?

 MYL2 is a contractile protein that plays a role in heart development and function. During cardiogenesis it plays an early role in cardiac contractility by promoting cardiac myofibril assembly and represents one of the earliest markers of ventricular specification (PMID 8506363).

 In general, MYL2 interacts with the neck/tail region of the muscle thick filament protein myosin to regulate myosin motility and function (PMID 8316858). Its N-terminal domain is responsible for calcium and magnesium binding at their activating concentrations. This induces a functionally important conformational change (PMID: 18202317). However, the ion dissociation rate is not fast enough to modulate cardiac contractility on a beat-by-beat basis (PMIDs: 188447, 8804617). In addition, RLC function can be modulated by posttranslational modifications such as phosphorylation and deamidation in the N-terminal region. As a result, a significant change in the charge of the protein is introduced, which undoubtedly alters its interaction with the C-terminal myosin alpha-helical domain (PMID: 26074085).

 What is MYL2's involvement in disease?

 Diseases associated with MYL2 dysfunction include forms of familial hypertrophic cardiomyopathies, congenital fiber-type disproportion, and heart failure (PMID: 26074085). 


实验方案

Product Specific Protocols
WB protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-APDownload protocol
IHC protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-APDownload protocol
IF protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-APDownload protocol
IP protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Science

Conversion of human fibroblasts into functional cardiomyocytes by small molecules.

Authors - Nan Cao
humanIF

Cell Stem Cell

Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells.

Authors - Lei Ye
humanFC

Cell Stem Cell

Pathogenic LMNA variants disrupt cardiac lamina-chromatin interactions and de-repress alternative fate genes.

Authors - Parisha P Shah
humanIF

Cell Stem Cell

Gene editing to prevent ventricular arrhythmias associated with cardiomyocyte cell therapy

Authors - Silvia Marchiano
pigIF

Cell Stem Cell

Gene editing to prevent ventricular arrhythmias associated with cardiomyocyte cell therapy

Authors - Silvia Marchiano
humanIF,FC

Nat Methods

Chemically defined generation of human cardiomyocytes.

Authors - Paul W Burridge