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  • Featured Product
  • KD/KO Validated

MYL5 Polyclonal antibody

MYL5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14249-1-AP

Print datasheet

Synonyms

MYL5, MyLC 2, MYLC2, Myosin light chain 5

验证数据展示

  • WB analysis using 14249-1-AP

    WB analysis using 14249-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14249-1-AP (MYL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 14249-1-AP

    IHC staining of human cervical cancer using 14249-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL5 antibody included
  • IHC staining of human cervical cancer using 14249-1-AP

    IHC staining of human cervical cancer using 14249-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL5 antibody included

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human brain tissue, rat skeletal muscle tissue
Positive IHC detected inhuman cervical cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYL5 Ready-To-Use IHC Kit. MYL5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14249-1-AP targets MYL5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MYL5 fusion protein Ag5522 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, light chain 5, regulatory
Synonyms MYL5, MyLC 2, MYLC2, Myosin light chain 5
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC040050
Gene Symbol MYL5
Gene ID (NCBI) 4636
RRIDAB_2266762
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02045
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYL5 antibody 14249-1-APDownload protocol
IHC protocol for MYL5 antibody 14249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Cytoskeleton (Hoboken)

The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division.

Authors - Ivan Ramirez
  • KD Validated
View Article