Mouse GM-CSF ELISA Kit0 Publications

Catalog number: KE10015

规格

单价

96 T

¥2600

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Overview


Product name:
Mouse GM-CSF ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
1.2 pg/mL

Range:
4.7 - 300 pg/mL

Reacted Species:
Mouse

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Cell culture supernatant 98% 85%-119%
Mouse serum 85% 80%-97%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 32.7 1.8 5.5
2 20 80.8 2.9 3.6
3 20 304.4 19.9 6.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 30.2 1.6 5.4
2 24 75.3 5.5 7.3
3 24 306.0 17.9 5.9

Product overview:
KE10015 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse GM-CSF. The assay recognizes mouse GM-CSF. A monoclonal antibody specific for mouse GM-CSF has been pre-coated onto the microwells. The mouse GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse GM-CSF is added to detect the captured mouse GM-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
Csf2, Csfgm, Gm CSf, GM-CSF, MGI IGM
Background

GM-CSF, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. GM-CSF is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. GM-CSF has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.


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