Mouse GM-CSF ELISA Kit

Sensitivity

1.2 pg/mL

Range

4.7-300 pg/mL

Reactivity

Mouse

Cat No : KE10015

说明书下载

Synonyms

Csf2, Csfgm, Gm CSf, GM-CSF, MGI IGM



Product Information

KE10015 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse GM-CSF. The assay recognizes mouse GM-CSF. An antibody specific for mouse GM-CSF has been pre-coated onto the microwells. The mouse GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse GM-CSF is added to detect the captured mouse GM-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse GM-CSF ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.2 pg/mL
Range 4.7-300 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 12981

Recovery

Sample TypeAverageRange
Mouse serum85%80%-97%
Cell culture supernatants98%85%-119%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12032.71.85.5
22080.82.93.6
320304.419.96.6

InterAssay

Samplenmean ( pg/mL)SDCV%
12430.21.65.4
22475.35.57.3
324306.017.95.9

Background Information

GM-CSF, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. GM-CSF is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. GM-CSF has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Csf2, Csfgm, Gm CSf, GM-CSF, MGI IGM

Publications

SpeciesSample TypeTitle
humancell culture supernatants

J Clin Invest

IL20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer

Authors - Yunfei He
mousetissue homogenates

Front Biosci (Landmark Ed)

Quercetin, the Ingredient of Xihuang Pills, Inhibits Hepatocellular Carcinoma by Regulating Autophagy and Macrophage Polarization

Authors - Ruoxia Wu